Detailed description of scanning electromagnetic microscope (SEM) of the Holothuria scabra’s ossicles (Holothuroidea: Echinodermata) collected from Pesawaran waters, Lampung, Indonesia

Abstract. Khatulistiani TS, Dewi AS, Yasman. 2022. Detailed description of scanning electromagnetic microscope (SEM) the Holothuria scabra’s ossicles (Holothuroidea: Echinodermata) collected from Pesawaran waters, Lampung, Indonesia. Biodiversitas 23: 3697-3704. Ossicles are microscopic calcified endoskeleton that unique across sea cucumber species. Furthermore, their integrity is maintained after physical and chemical processes make them a desired attribute for identification purposes. Understanding ossicle characteristic especially sandfish H. scabra juvenilles important during mariculture prevent fraud due to high economic value. This study aims identify wild Lampung Province, Indonesia using electron microscopes get more detailed morphology ossicles. Seven specimens were in Pesawaran, Sari Ringgung Mutun, around waters. Morphology carried out by observing body appearance tentacles as well measuring weights lengths. The observation was performed light (LEICA ICC50 HD) at 400x magnification SEM (Jeol-JCM6000). length tested 140-300 mm weight approximately 99-579 g. These two parameters found exhibit positive correlation (R2 = 0.93; R= 0.96; p 0.000). All observed displayed peltate (12-22 tentacles) surrounding mouths. between tentacle numbers also (R2= 0.53; 0.73; 0.101). shape consist six fourteen-hole buttons, tables, spiny perforated plates cloaca, tube foot rods, dorsal I shaped branched rods.